Coding

Part:BBa_K2082209

Designed by: Pascal Schmidt   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-07)


Fusion protein Gal11P:cI(434)

Gal11P:cI(434) fusion protein- BBa_K2082209

This BioBrick contains a fusion protein of the regulatory protein Gal11P of Saccharomyces cerevisae and the repressor protein cI of the phage 434. Together with the part BBa_K2082226 it can be used as a positive control for the bacterial two-hybrid system. The interaction of the Gal4 protein with Gal11P is well documented (Jeong et al. 2001). Due to the fact that we had some time issues at the end, the functionality of this positive control could not be tested. The generator of this fusion protein is the BioBrick BBa_K2082227

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 522
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None